{"id":1423,"date":"2023-11-14T18:43:41","date_gmt":"2023-11-14T18:43:41","guid":{"rendered":"https:\/\/thescienceteacher.ie\/?p=1423"},"modified":"2023-11-14T18:58:05","modified_gmt":"2023-11-14T18:58:05","slug":"nutrient-agar-plates","status":"publish","type":"post","link":"https:\/\/thescienceteacher.ie\/?p=1423","title":{"rendered":"Nutrient Agar Plates"},"content":{"rendered":"\n
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I made up nutrient agar plates for my 3rd years last week. At the start of the microorganisms chapter I gave each student a plate. They divided it into three sections using a sharpie and labelled each section. They then used sterile swaps to swap three surfaces. Students could decided what they wanted to swap. I suggested their hands, phones, door handles…<\/p>\n\n\n\n

We incubated the plates and viewed them at the end of the week when we were finishing up the theory. They were HORRIFIED!!<\/p>\n\n\n\n

I followed the following method (It worked perfectly for me and grew microbes but everyone has their own way)<\/p>\n\n\n\n

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  1. Placed 24g of nutrient agar into two conical flasks.<\/li>\n\n\n\n
  2. I added 500ml of boiling water into each flask, stirring well for a few minutes.<\/li>\n\n\n\n
  3. I placed cotton wool to stopped the flasks and placed both onto a hot plate.<\/li>\n\n\n\n
  4. Continue to stir every couple of minutes to ensure the agar isn’t clumped at the bottom.<\/li>\n\n\n\n
  5. Put on a low heat as it will boil over quickly as it was boiling water that was added.<\/li>\n\n\n\n
  6. I simmered the mixtures for approximately 10 minutes.<\/li>\n\n\n\n
  7. Poured straight into petri dishes.<\/li>\n\n\n\n
  8. Cooled and stored in the fridge.<\/li>\n\n\n\n
  9. This made 24 plates.<\/li>\n<\/ol>\n\n\n\n

    Lots of you asked how I made my plates so I hope this helps you out!<\/p>\n<\/div>\n\n\n\n

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    Method for making up nutrient agar plates.<\/p>\n","protected":false},"author":5,"featured_media":1428,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,45],"tags":[],"class_list":["post-1423","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-junior-cycle"],"_links":{"self":[{"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/posts\/1423","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1423"}],"version-history":[{"count":3,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/posts\/1423\/revisions"}],"predecessor-version":[{"id":1435,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/posts\/1423\/revisions\/1435"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=\/wp\/v2\/media\/1428"}],"wp:attachment":[{"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1423"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1423"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/thescienceteacher.ie\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}